Abstract
Porcine deltacoronavirus (PDCoV) is an emerging coronavirus in humans and already prominent virus infecting and killing many pigs every year. The need to investigate possible therapeutic targets to prevent the infection or proliferation of PDCoV is becoming more and more crucial. BIRC3 was identified as a possible therapeutic target as it is a host gene significantly upregulated during PDCoV infection. Two BIRC3 knockout swine testicular (ST) cell lines were established by transducing a lentiviral vector into ST cells, these cells were selected for using puromycin as the lentiviral vector contains a puromycin resistant gene. The BIRC3 knockout cell lines and wild type cells were infected with PDCoV for 48 hours. The cells and the supernatant were harvested every 12 hours and RNA extraction was carried out before qRT-PCR. Significant results were seen at 48 hours post-infection of both BIRC3 knockout cell lines compared to wild type cells. To solidify the results are consistent with a BIRC3 knockout, the BIRC3 knockout cell lines were compared to each other. The knockout lines were not statistically significant compared to each other indicating the results at 48 hours post-infection seen comparing the wild type and BIRC3 knockout lines are a result of the BIRC3 knockout.
Advisor
Evans, Ellyn
Department
Biochemistry and Molecular Biology
Recommended Citation
Georskey, Hallie, "The Impact BIRC3 has on Porcine Deltacoronavirus Infection in a Swine Testicular Cell Line" (2024). Senior Independent Study Theses. Paper 11171.
https://openworks.wooster.edu/independentstudy/11171
Disciplines
Biochemistry | Molecular Biology | Virology
Publication Date
2024
Degree Granted
Bachelor of Arts
Document Type
Senior Independent Study Thesis
© Copyright 2024 Hallie Georskey