Characterization of Putative Molecule Inhibitors of MurG and Their Effectiveness in Growth Inhibition of Escherichia coli (DH5⍺), Pseudomonas frederikbergensis (36C6) and Pseudomonas chlororaphis (14B11)

Authors

Spencer GabrielFollow

Abstract

MurG is an enzyme responsible for catalyzing the formation of the glycosidic bond that forms Lipid II from Lipid I, so that it can be transported out of the cell to form the cell wall.¹ The inhibition of this enzyme would be detrimental to the life of the cell as the cell wall provides necessary support and protection to the bacterial cell.^2,3 Past work has provided insight to inhibitors including important structural components of inhibitors of MurG but none have provided a quality inhibitor that is able to work in vivo.^4–6 Work in the Martin lab has led to potential inhibitors that remain untested for bacterial growth inhibition.^7–9 The compounds were characterized using proton and carbon Nuclear Magnetic Resonance (NMR) and Infrared Spectroscopy (IR). Microdilution assays were done to determine and MIC of the compound in which Compounds 7 and 8 did not have any antibiotic activity against Escherichia coli (DH5a), Pseudomonas frederikbergensis (36C6) and Pseudomonas chlororaphis (14B11) within the assessed concentration range.

Advisor

Martin, Sara

Department

Biochemistry and Molecular Biology

Recommended Citation

Gabriel, Spencer, "Characterization of Putative Molecule Inhibitors of MurG and Their Effectiveness in Growth Inhibition of Escherichia coli (DH5⍺), Pseudomonas frederikbergensis (36C6) and Pseudomonas chlororaphis (14B11)" (2024). Senior Independent Study Theses. Paper 11126.
https://openworks.wooster.edu/independentstudy/11126

Publication Date

2024

Degree Granted

Bachelor of Arts

Document Type

Senior Independent Study Thesis