Abstract
The ability to detect neurotransmitter release and concentration at the synapse is of particular importance in understanding the pathology of neuronal disorders. Cellular based neurotransmitter fluorescently engineered reporters (CNiFERs) are a recently developed tool to image neurotransmitter release with high temporal and spatial resolution. Developing these reporters in yeast may provide a cheaper and more flexible alternative to using mammalian cell lines for reporter expression. Measurements of plasmids from two transformations in E. Coli were confirmed, and transformation of yeast was successful. Further work is needed to test the fluorescent activity of the engineered reporter. Some literature suggests that yeast may not be suitable for neurotransmitter sensing. Future work may use the yeast strain for investigating affinities and kinetics of potential ligands.
Advisor
Morgan, William
Department
Neuroscience
Recommended Citation
McMichael, William Valentine, "Developing Yeast As A Cellular Based Neurotransmitter Flourescently Engineered Reporter (Cnifer) For Acetylcholine" (2022). Senior Independent Study Theses. Paper 9911.
https://openworks.wooster.edu/independentstudy/9911
Disciplines
Biology | Cellular and Molecular Physiology | Genetics
Publication Date
2022
Degree Granted
Bachelor of Arts
Document Type
Senior Independent Study Thesis
© Copyright 2022 William Valentine McMichael