Abstract
The goal of this research is to determine if the secretome of cardiomyocytes derived from induced pluripotent stem cells (iPSCs) can be used to determine potential biomarkers of Leigh syndrome (LS) from the excreted metabolic profile of diseased cells. Small molecules in depleted media from cardiomyocytes were measured using liquid chromatography-quadrupole time-of-flight-mass spectrometry (LC-QTOF-MS) to determine prospective biomarkers of a mitochondrial disease known as LS. LC-QTOF-MS was used to compare the metabolic profiles of 4 LS samples to 4 control group cardiomyocytes to determine metabolites unique from the non-depleted media. Small molecule metabolites unique from the depleted cell culture media were identified using the MS/MS fragmentation pattern. Glutathione and pyrroline hydroxycarboxylic acid were identified in the depleted media LS samples. The project determined that the secretome from iPSCs can aid in our understanding of metabolomics in relation to diseases like Leigh syndrome, however there are still many challenges to overcome including: the large number of background molecules and in complete metabolite libraries.
Advisor
Edmiston, Paul
Department
Biochemistry and Molecular Biology
Recommended Citation
Painter, Eve, "Profiling the Secretome of iPSC Cardiomyocytes Using High Resolution-Mass Spectrometry for Leigh Syndrome Biomarker Discovery" (2024). Senior Independent Study Theses. Paper 11139.
https://openworks.wooster.edu/independentstudy/11139
Disciplines
Analytical, Diagnostic and Therapeutic Techniques and Equipment | Biochemical Phenomena, Metabolism, and Nutrition | Congenital, Hereditary, and Neonatal Diseases and Abnormalities | Diagnosis | Disease Modeling | Other Analytical, Diagnostic and Therapeutic Techniques and Equipment
Keywords
Biomarker, iPSCs, LC-QTOF-MS
Publication Date
2024
Degree Granted
Bachelor of Arts
Document Type
Senior Independent Study Thesis
© Copyright 2024 Eve Painter